Device

Part:BBa_K5477042:Design

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-27)


Biosensor device II for detection of PAHs, dioxin or dioxin-like PCBs


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 2718
    Illegal EcoRI site found at 3824
    Illegal EcoRI site found at 5856
    Illegal EcoRI site found at 6478
    Illegal EcoRI site found at 9170
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
    Illegal SpeI site found at 1896
    Illegal SpeI site found at 3668
    Illegal SpeI site found at 4059
    Illegal SpeI site found at 5305
    Illegal SpeI site found at 5606
    Illegal PstI site found at 5365
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 2718
    Illegal EcoRI site found at 3824
    Illegal EcoRI site found at 5856
    Illegal EcoRI site found at 6478
    Illegal EcoRI site found at 9170
    Illegal NheI site found at 869
    Illegal NheI site found at 2090
    Illegal NheI site found at 4253
    Illegal NheI site found at 7618
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
    Illegal SpeI site found at 1896
    Illegal SpeI site found at 3668
    Illegal SpeI site found at 4059
    Illegal SpeI site found at 5305
    Illegal SpeI site found at 5606
    Illegal PstI site found at 5365
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 2718
    Illegal EcoRI site found at 3824
    Illegal EcoRI site found at 5856
    Illegal EcoRI site found at 6478
    Illegal EcoRI site found at 9170
    Illegal BglII site found at 102
    Illegal BglII site found at 1879
    Illegal BamHI site found at 7120
    Illegal BamHI site found at 9114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 2718
    Illegal EcoRI site found at 3824
    Illegal EcoRI site found at 5856
    Illegal EcoRI site found at 6478
    Illegal EcoRI site found at 9170
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
    Illegal SpeI site found at 1896
    Illegal SpeI site found at 3668
    Illegal SpeI site found at 4059
    Illegal SpeI site found at 5305
    Illegal SpeI site found at 5606
    Illegal PstI site found at 5365
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1064
    Illegal EcoRI site found at 2718
    Illegal EcoRI site found at 3824
    Illegal EcoRI site found at 5856
    Illegal EcoRI site found at 6478
    Illegal EcoRI site found at 9170
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
    Illegal SpeI site found at 1896
    Illegal SpeI site found at 3668
    Illegal SpeI site found at 4059
    Illegal SpeI site found at 5305
    Illegal SpeI site found at 5606
    Illegal PstI site found at 5365
    Illegal AgeI site found at 1945
    Illegal AgeI site found at 4108
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use to detect PAHs, dioxin and dioxin-like PCBs in breast milk. The different composites that are receptor modules were cloned in YCp backbones while the reporter module was cloned in pUUS backbone.


Source

Homo sapiens, S. cerevisiae and E. coli


References